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Culture system containing PBMTM Neural Progenitor Basal Medium (CC-3210), NPMMTM Maintenance Media SingleQuotsTM (CC-4241) and Neural Progenitor Supplement SingleQuotsTM (CC-4242) required for growth and differentiation of neural progenitor cells
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Image Search Results
Journal: iScience
Article Title: ApoE maintains neuronal integrity via microRNA and H3K27me3-mediated repression
doi: 10.1016/j.isci.2024.109231
Figure Lengend Snippet: In vitro differentiated neurons recapitulate key transcriptional signatures of neurotypes identified from single-cell RNA-sequencing of human ventral midbrain (A) Differentiation of neuronal stem cells (NSCs) into neural progenitor cells (NPCs) and mature neurons (Day 14), followed by 3 additional weeks of culture. Cells were harvested on different day “D” for experiment. (B) Measurement of ApoE and MAP2 mRNA level by quantitative PCR (qPCR) in isogenic control and ApoE−/− cells harvested on different day. Data are presented as mean ± S.D. (triplicate qPCR reactions, 2-tailed t -tests). (C) Representative immunoblots and MAP2 quantification of cells harvested on different days. Lysates were probed with MAP2, actin and ApoE antibodies. The level of MAP2 was normalized to actin and quantified with reference to Day 14 control neurons. Data presented as mean ± S.E.M (∗∗p < 0.005, n = 5. 2-tailed paired t -test). (D) Representative immmunofluorescence images of Day 14 neurons stained with MAP2 and DAPI. The scale bars represent 150 μm. (E) RNA-seq volcano plots of differentially expressed genes between NSC and Day 14 neurons as determined by DESeq2 with Log 2 (2-fold change) threshold and adjusted p-value <0.05. (F) Top 10 enriched gene ontology terms (BP: Biological Process) for genes highly expressed in either control or ApoE−/− neurons (left) and NSC (right). (G) Ranked enrichment analysis of gene expression signatures in control and ApoE−/− cells (Negative: NSC; Positive: Neurons) by directional fgsea statistical enrichment test using cell type signature gene sets. “h” indicates human; GABA: GABAergic neurons; CTX: cerebral cortex; DA: dopaminergic neurons; SERT: serotonergic; NB: neuroblast; NbML: mediolateral neuroblasts; OMTN: oculomotor and trochlear nucleus; RN: red nucleus; OPC: oligodendrocyte precursor cells; PROG: Progenitor; FPL: lateral floorplate; BP: basal plate; FPM: medial floorplate; ENDO: endothelial cells; PFC: prefrontal cortex.
Article Snippet:
Techniques: In Vitro, RNA Sequencing, Real-time Polymerase Chain Reaction, Control, Western Blot, Staining, Gene Expression
Journal: iScience
Article Title: ApoE maintains neuronal integrity via microRNA and H3K27me3-mediated repression
doi: 10.1016/j.isci.2024.109231
Figure Lengend Snippet:
Article Snippet:
Techniques: Recombinant, Transfection, Reverse Transcription, Imaging, Expressing, Control, Knock-Out, Sequencing, Negative Control, Plasmid Preparation, Software, Functional Assay